Review




Structured Review

Proteintech cd31
mGFD‐CD3 induces hepatotoxicity by targeting hepatic endothelial cells in mice. (A) H&E analysis performed at 24 h post‐injection of BiTE in the liver tissues of naturally aged mice. Scale bar, 30 μm (original) and 15 μm (magnified). (B) Immunofluorescence analysis was conducted on liver, lung, and kidney samples from naturally aged mice collected 24 h post‐BiTE injection ( n = 6 view field per each treatment group). Scale bar, 30 μm. (C) Immunofluorescence analysis of liver samples of naturally aged mice taken 4 h post‐injection of BiTE to examine the colocalization of BiTE, <t>CD31,</t> and uPAR. Scale bar, 30 μm (original) and 15 μm (magnified). (D) Immunofluorescence analysis to examine the colocalization of CD31 and uPAR in the liver tissues of wild type mice (WT) and uPAR knockout mice; ( n = 3 mice per treatment group). Scale bar, 30 μm. (E) Levels of AST and ALT in serum samples from mice before and after BiTE injection were determined with a Biochemical Analyzer; ( n = 3 mice per treatment group). Data is presented as mean ± SD and analyzed by unpaired two‐tailed Student's t ‐test with Welch's correction. *** p ≤ 0.001.
Cd31, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd31+antibody/Cd31+Polyclonal+antibody/pmc13620297-381-14-15
Average 96 stars, based on 262 article reviews
cd31 - by Bioz Stars, 2026-10
96/100 stars

Images

1) Product Images from "uPAR ‐Targeting T Cell Engager Exerts Senolytic Effects in Mice and Non‐Human Primates With Serum Aminotransferase Activity as a Safety Monitor"

Article Title: uPAR ‐Targeting T Cell Engager Exerts Senolytic Effects in Mice and Non‐Human Primates With Serum Aminotransferase Activity as a Safety Monitor

Journal: Aging Cell

doi: 10.1111/acel.70742

mGFD‐CD3 induces hepatotoxicity by targeting hepatic endothelial cells in mice. (A) H&E analysis performed at 24 h post‐injection of BiTE in the liver tissues of naturally aged mice. Scale bar, 30 μm (original) and 15 μm (magnified). (B) Immunofluorescence analysis was conducted on liver, lung, and kidney samples from naturally aged mice collected 24 h post‐BiTE injection ( n = 6 view field per each treatment group). Scale bar, 30 μm. (C) Immunofluorescence analysis of liver samples of naturally aged mice taken 4 h post‐injection of BiTE to examine the colocalization of BiTE, CD31, and uPAR. Scale bar, 30 μm (original) and 15 μm (magnified). (D) Immunofluorescence analysis to examine the colocalization of CD31 and uPAR in the liver tissues of wild type mice (WT) and uPAR knockout mice; ( n = 3 mice per treatment group). Scale bar, 30 μm. (E) Levels of AST and ALT in serum samples from mice before and after BiTE injection were determined with a Biochemical Analyzer; ( n = 3 mice per treatment group). Data is presented as mean ± SD and analyzed by unpaired two‐tailed Student's t ‐test with Welch's correction. *** p ≤ 0.001.
Figure Legend Snippet: mGFD‐CD3 induces hepatotoxicity by targeting hepatic endothelial cells in mice. (A) H&E analysis performed at 24 h post‐injection of BiTE in the liver tissues of naturally aged mice. Scale bar, 30 μm (original) and 15 μm (magnified). (B) Immunofluorescence analysis was conducted on liver, lung, and kidney samples from naturally aged mice collected 24 h post‐BiTE injection ( n = 6 view field per each treatment group). Scale bar, 30 μm. (C) Immunofluorescence analysis of liver samples of naturally aged mice taken 4 h post‐injection of BiTE to examine the colocalization of BiTE, CD31, and uPAR. Scale bar, 30 μm (original) and 15 μm (magnified). (D) Immunofluorescence analysis to examine the colocalization of CD31 and uPAR in the liver tissues of wild type mice (WT) and uPAR knockout mice; ( n = 3 mice per treatment group). Scale bar, 30 μm. (E) Levels of AST and ALT in serum samples from mice before and after BiTE injection were determined with a Biochemical Analyzer; ( n = 3 mice per treatment group). Data is presented as mean ± SD and analyzed by unpaired two‐tailed Student's t ‐test with Welch's correction. *** p ≤ 0.001.

Techniques Used: Analysis, Injection, Immunofluorescence, Knock-Out, Blood/Plasma/Serum Samples, Two Tailed Test

Related Articles

Staining:

Article Title: A novel molecule ZYZ329 targeting histone methyltransferase SMYD3 suppresses pathological angiogenesis via the driven mitochondrial ROS/HIF-1α/VEGFA axis
Article Snippet: .. We systematically selected five non-consecutive sections per plug for immunofluorescent staining with an anti-CD31 antibody (1:400, 28083-1-AP, Proteintech) and DAPI. .. Images were captured on an Olympus SpinSR10 confocal microscope using a 20× objective.

Article Title: A novel molecule ZYZ329 targeting histone methyltransferase SMYD3 suppresses pathological angiogenesis via the driven mitochondrial ROS/HIF-1α/VEGFA axis.
Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS non-consecutive sections per plug for immunofluorescent staining with an anti-CD31 antibody (1:400, 28083-1-AP, Proteintech) and DAPI. .. Images were captured on an Olympus SpinSR10 confocal microscope using a 20× objective.

Article Title: Insufficient Erythrocyte-derived S1P:A Pathogenic Driver and Diagnostic Biolipid for Tumor Progression
Article Snippet: .. 836 Immunofluorescence staining 837 Jo urn al Pr e-p roo f 39 To assess microvessel density, tumor sections were stained with an anti-CD31 antibody (Proteintech, 838 28083-1-AP). ..

Article Title: Insufficient erythrocyte-derived S1P: A pathogenic driver and diagnostic biolipid for tumor progression
Article Snippet: .. To assess microvessel density, tumor sections were stained with an anti-CD31 antibody (Proteintech, 28083-1-AP). ..

Incubation:

Article Title: Danqi Pill (DQP) Attenuates OGD-Induced Injury in HUVECs via SHP2-ROS-NLRP3 Axis.
Article Snippet: Background: The NLRP3 inflammasomemediated inflammatory response plays a critical role in endothelial dysfunction.. Danqi pill (DQP), a Chinese patent medicine composed of Salvia miltiorrhiza and Panax notoginseng, has demonstrated protective effects on endothelial cells, though its underlying mechanisms remain incompletely understood.. Methods: Human umbilical vein endothelial cells (HUVECs) were subjected to oxygenglucose deprivation (OGD) to simulate ischemic injury.

Article Title: Elucidating the protective mechanisms of umbilical cord mesenchymal stem cells against stenosis-induced deep venous thrombosis during pregnancy: a transcriptomic and metabolomic study
Article Snippet: .. Subsequently, cells were incubated at 4 °C with anti-CD34 antibody (144860-1-AP, Proteintech), or anti-CD31 antibody (66065-2-Ig, Proteintech), followed by incubation with a Cy3-conjugated secondary antibody (SA00009-2, Proteintech) for 1 h at room temperature. ..

Article Title: Elucidating the protective mechanisms of umbilical cord mesenchymal stem cells against stenosis-induced deep venous thrombosis during pregnancy: a transcriptomic and metabolomic study
Article Snippet: .. After dewaxing, rehydration, and antigen retrieval, the sections were incubated with anti-CD34 or anti-CD31 antibody (1:1000, Proteintech). ..

Immunofluorescence:

Article Title: Insufficient Erythrocyte-derived S1P:A Pathogenic Driver and Diagnostic Biolipid for Tumor Progression
Article Snippet: .. 836 Immunofluorescence staining 837 Jo urn al Pr e-p roo f 39 To assess microvessel density, tumor sections were stained with an anti-CD31 antibody (Proteintech, 838 28083-1-AP). ..

other:

Article Title: Insufficient erythrocyte-derived S1P: A pathogenic driver and diagnostic biolipid for tumor progression
Article Snippet: Anti-CD31 antibody , Proteintech , Cat# 28083-1-AP; RRID: AB_2881055.



Similar Products

98
R&D Systems anti cd31 antibody
Anti Cd31 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd31+antibody/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pmc13621939-56-29-31
Average 98 stars, based on 1 article reviews
anti cd31 antibody - by Bioz Stars, 2026-10
98/100 stars
  Buy from Supplier

98
R&D Systems updated february 2025 goat anti cd31
Updated February 2025 Goat Anti Cd31, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd31+antibody/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pm42809441-78-0-6
Average 98 stars, based on 1 article reviews
updated february 2025 goat anti cd31 - by Bioz Stars, 2026-10
98/100 stars
  Buy from Supplier

96
Proteintech cd31
mGFD‐CD3 induces hepatotoxicity by targeting hepatic endothelial cells in mice. (A) H&E analysis performed at 24 h post‐injection of BiTE in the liver tissues of naturally aged mice. Scale bar, 30 μm (original) and 15 μm (magnified). (B) Immunofluorescence analysis was conducted on liver, lung, and kidney samples from naturally aged mice collected 24 h post‐BiTE injection ( n = 6 view field per each treatment group). Scale bar, 30 μm. (C) Immunofluorescence analysis of liver samples of naturally aged mice taken 4 h post‐injection of BiTE to examine the colocalization of BiTE, <t>CD31,</t> and uPAR. Scale bar, 30 μm (original) and 15 μm (magnified). (D) Immunofluorescence analysis to examine the colocalization of CD31 and uPAR in the liver tissues of wild type mice (WT) and uPAR knockout mice; ( n = 3 mice per treatment group). Scale bar, 30 μm. (E) Levels of AST and ALT in serum samples from mice before and after BiTE injection were determined with a Biochemical Analyzer; ( n = 3 mice per treatment group). Data is presented as mean ± SD and analyzed by unpaired two‐tailed Student's t ‐test with Welch's correction. *** p ≤ 0.001.
Cd31, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd31+antibody/Cd31+Polyclonal+antibody/pmc13620297-381-14-15
Average 96 stars, based on 1 article reviews
cd31 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

94
Elabscience Biotechnology pe cyanine7 antimouse cd31 antibody
mGFD‐CD3 induces hepatotoxicity by targeting hepatic endothelial cells in mice. (A) H&E analysis performed at 24 h post‐injection of BiTE in the liver tissues of naturally aged mice. Scale bar, 30 μm (original) and 15 μm (magnified). (B) Immunofluorescence analysis was conducted on liver, lung, and kidney samples from naturally aged mice collected 24 h post‐BiTE injection ( n = 6 view field per each treatment group). Scale bar, 30 μm. (C) Immunofluorescence analysis of liver samples of naturally aged mice taken 4 h post‐injection of BiTE to examine the colocalization of BiTE, <t>CD31,</t> and uPAR. Scale bar, 30 μm (original) and 15 μm (magnified). (D) Immunofluorescence analysis to examine the colocalization of CD31 and uPAR in the liver tissues of wild type mice (WT) and uPAR knockout mice; ( n = 3 mice per treatment group). Scale bar, 30 μm. (E) Levels of AST and ALT in serum samples from mice before and after BiTE injection were determined with a Biochemical Analyzer; ( n = 3 mice per treatment group). Data is presented as mean ± SD and analyzed by unpaired two‐tailed Student's t ‐test with Welch's correction. *** p ≤ 0.001.
Pe Cyanine7 Antimouse Cd31 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd31+antibody/PE%2FCyanine7+Anti-Mouse+CD31+Antibody/10__1038_slash_s41467___026___78085___9-465-0-30
Average 94 stars, based on 1 article reviews
pe cyanine7 antimouse cd31 antibody - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

92
Elabscience Biotechnology e ab f1180b anti mouse cd45 2 elabscience
mGFD‐CD3 induces hepatotoxicity by targeting hepatic endothelial cells in mice. (A) H&E analysis performed at 24 h post‐injection of BiTE in the liver tissues of naturally aged mice. Scale bar, 30 μm (original) and 15 μm (magnified). (B) Immunofluorescence analysis was conducted on liver, lung, and kidney samples from naturally aged mice collected 24 h post‐BiTE injection ( n = 6 view field per each treatment group). Scale bar, 30 μm. (C) Immunofluorescence analysis of liver samples of naturally aged mice taken 4 h post‐injection of BiTE to examine the colocalization of BiTE, <t>CD31,</t> and uPAR. Scale bar, 30 μm (original) and 15 μm (magnified). (D) Immunofluorescence analysis to examine the colocalization of CD31 and uPAR in the liver tissues of wild type mice (WT) and uPAR knockout mice; ( n = 3 mice per treatment group). Scale bar, 30 μm. (E) Levels of AST and ALT in serum samples from mice before and after BiTE injection were determined with a Biochemical Analyzer; ( n = 3 mice per treatment group). Data is presented as mean ± SD and analyzed by unpaired two‐tailed Student's t ‐test with Welch's correction. *** p ≤ 0.001.
E Ab F1180b Anti Mouse Cd45 2 Elabscience, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd31+antibody/Biotin+Anti-Mouse+CD31+Antibody/pm42773266-850-85-88
Average 92 stars, based on 1 article reviews
e ab f1180b anti mouse cd45 2 elabscience - by Bioz Stars, 2026-10
92/100 stars
  Buy from Supplier

95
Novus Biologicals antibodies against cd31
mGFD‐CD3 induces hepatotoxicity by targeting hepatic endothelial cells in mice. (A) H&E analysis performed at 24 h post‐injection of BiTE in the liver tissues of naturally aged mice. Scale bar, 30 μm (original) and 15 μm (magnified). (B) Immunofluorescence analysis was conducted on liver, lung, and kidney samples from naturally aged mice collected 24 h post‐BiTE injection ( n = 6 view field per each treatment group). Scale bar, 30 μm. (C) Immunofluorescence analysis of liver samples of naturally aged mice taken 4 h post‐injection of BiTE to examine the colocalization of BiTE, <t>CD31,</t> and uPAR. Scale bar, 30 μm (original) and 15 μm (magnified). (D) Immunofluorescence analysis to examine the colocalization of CD31 and uPAR in the liver tissues of wild type mice (WT) and uPAR knockout mice; ( n = 3 mice per treatment group). Scale bar, 30 μm. (E) Levels of AST and ALT in serum samples from mice before and after BiTE injection were determined with a Biochemical Analyzer; ( n = 3 mice per treatment group). Data is presented as mean ± SD and analyzed by unpaired two‐tailed Student's t ‐test with Welch's correction. *** p ≤ 0.001.
Antibodies Against Cd31, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd31+antibody/CD31%2FPECAM-1+Antibody+(JC%2F70A)+-+BSA+Free/10__1038_slash_s41467___026___77738___z-547-23-28
Average 95 stars, based on 1 article reviews
antibodies against cd31 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

98
R&D Systems cd31
ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and <t>CD31</t> (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.
Cd31, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd31+antibody/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pmc13091142-299-20-21
Average 98 stars, based on 1 article reviews
cd31 - by Bioz Stars, 2026-10
98/100 stars
  Buy from Supplier

95
ABclonal Biotechnology anti cd31
ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and <t>CD31</t> (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.
Anti Cd31, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd31+antibody/CD31%2FPECAM1+Rabbit+mAb/pmc13561949-51-41-42
Average 95 stars, based on 1 article reviews
anti cd31 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

95
ABclonal Biotechnology rabbit anti cd31
ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and <t>CD31</t> (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.
Rabbit Anti Cd31, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd31+antibody/CD31%2FPECAM1+Rabbit+mAb/pm42660379-100-15-19
Average 95 stars, based on 1 article reviews
rabbit anti cd31 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

96
Proteintech anti cd31
ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and <t>CD31</t> (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.
Anti Cd31, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd31+antibody/Cd31+Polyclonal+antibody/pmc13655877-87-18-21
Average 96 stars, based on 1 article reviews
anti cd31 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

Image Search Results


mGFD‐CD3 induces hepatotoxicity by targeting hepatic endothelial cells in mice. (A) H&E analysis performed at 24 h post‐injection of BiTE in the liver tissues of naturally aged mice. Scale bar, 30 μm (original) and 15 μm (magnified). (B) Immunofluorescence analysis was conducted on liver, lung, and kidney samples from naturally aged mice collected 24 h post‐BiTE injection ( n = 6 view field per each treatment group). Scale bar, 30 μm. (C) Immunofluorescence analysis of liver samples of naturally aged mice taken 4 h post‐injection of BiTE to examine the colocalization of BiTE, CD31, and uPAR. Scale bar, 30 μm (original) and 15 μm (magnified). (D) Immunofluorescence analysis to examine the colocalization of CD31 and uPAR in the liver tissues of wild type mice (WT) and uPAR knockout mice; ( n = 3 mice per treatment group). Scale bar, 30 μm. (E) Levels of AST and ALT in serum samples from mice before and after BiTE injection were determined with a Biochemical Analyzer; ( n = 3 mice per treatment group). Data is presented as mean ± SD and analyzed by unpaired two‐tailed Student's t ‐test with Welch's correction. *** p ≤ 0.001.

Journal: Aging Cell

Article Title: uPAR ‐Targeting T Cell Engager Exerts Senolytic Effects in Mice and Non‐Human Primates With Serum Aminotransferase Activity as a Safety Monitor

doi: 10.1111/acel.70742

Figure Lengend Snippet: mGFD‐CD3 induces hepatotoxicity by targeting hepatic endothelial cells in mice. (A) H&E analysis performed at 24 h post‐injection of BiTE in the liver tissues of naturally aged mice. Scale bar, 30 μm (original) and 15 μm (magnified). (B) Immunofluorescence analysis was conducted on liver, lung, and kidney samples from naturally aged mice collected 24 h post‐BiTE injection ( n = 6 view field per each treatment group). Scale bar, 30 μm. (C) Immunofluorescence analysis of liver samples of naturally aged mice taken 4 h post‐injection of BiTE to examine the colocalization of BiTE, CD31, and uPAR. Scale bar, 30 μm (original) and 15 μm (magnified). (D) Immunofluorescence analysis to examine the colocalization of CD31 and uPAR in the liver tissues of wild type mice (WT) and uPAR knockout mice; ( n = 3 mice per treatment group). Scale bar, 30 μm. (E) Levels of AST and ALT in serum samples from mice before and after BiTE injection were determined with a Biochemical Analyzer; ( n = 3 mice per treatment group). Data is presented as mean ± SD and analyzed by unpaired two‐tailed Student's t ‐test with Welch's correction. *** p ≤ 0.001.

Article Snippet: All primary antibodies used in this work were commercially available: uPAR (Proteintech, 10286‐1‐AP, 1:200), CD31 (Proteintech, 28083‐1‐AP, 1:400), F4/80 (CST, 70076S, 1:200), and Fc (Invitrogen, A18818, 1:200).

Techniques: Analysis, Injection, Immunofluorescence, Knock-Out, Blood/Plasma/Serum Samples, Two Tailed Test

ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and CD31 (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.

Journal: Bioactive Materials

Article Title: Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration

doi: 10.1016/j.bioactmat.2026.04.004

Figure Lengend Snippet: ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and CD31 (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.

Article Snippet: After antigen retrieval and blocking, sections were incubated overnight at 4 °C with primary antibodies targeting vimentin, CD68 (ABclonal, A20803), CD31 (R&D SYSTEMS, AF3628), HMGB1 (Cell Signaling Technology, 3935), HSP70 (ABclonal, A23457), NF-κB p65 (ABclonal, A19653), CD206 (Cell Signaling Technology, 24595), and FAPα (ABclonal, A23789 ).

Techniques: Immunofluorescence, Expressing, Marker